◂ PEPTIDE GLOSSARY
Biochemistry

Secondary & Tertiary Structure

Also known as: primary structure · secondary structure · tertiary structure · quaternary structure · protein folding

The levels of peptide/protein organisation: sequence (primary), local folds (secondary), 3-D shape (tertiary), assembly (quaternary).

A peptide or protein is described at several structural levels. Primary structure is the amino-acid sequence itself. Secondary structure is local, repeating folding — alpha-helices and beta-sheets — held by hydrogen bonds. Tertiary structure is the overall three-dimensional shape of one chain. Quaternary structure is how multiple chains assemble.

Short peptides may have little fixed secondary or tertiary structure, but even a small fold can be essential to activity. Shape follows directly from sequence, which is why a single substituted residue can change how a molecule works.

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